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Bioptechs inc fcs2 stage adapter
Fcs2 Stage Adapter, supplied by Bioptechs inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fcs2+stage+adapter/FCS2+Cooling+Adapter/pm41990751-538-15-18
Average 96 stars, based on 1 article reviews
fcs2 stage adapter - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Imaging:

Article Title: Epigenetic plasticity cooperates with cell-cell interactions to direct pancreatic tumorigenesis
Article Snippet: .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. Next, we flowed imaging buffer into the sample and generated a low magnification map of the entire tissue using a 20X Plan APO objective (Nikon, MRD00205).

Article Title: Fast Relaxation Imaging in living cells.
Article Snippet: This protocol describes the technique of Fast Relaxation Imaging (FReI) as applied to protein folding inside living cells.. The required modifications of a fluorescence microscope by addition of a diode laser temperature jump source, a yellow/blue switchable lightemitting diode source, and a two-color CCD camera to collect movies of protein dynamics inside cells are discussed.. A description of how proteins are labeled for imaging, how cells are prepared for imaging, and how imaging of kinetics inside cells with millisecond time resolution is obtained, along with the complementary in vitro experiments, is also provided.

Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor niche at the benign-to-malignant transition.
Article Snippet: Cleaned coverslips were coated with a silane layer to allow stabilization of a polyacrylamide gel during smFISH staining, following published protocols111: they were submerged in 0.1% (vol/vol) triethylamine (Millipore, TX1200) and 0.2% (vol/vol) allyltrichlorosilane (Sigma, 107778) in chloroform for 30 min at room temperature, washed once with chloroform, washed once with 100% ethanol and dried using nitrogen gas. .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. To prepare for staining individual samples, silanized coverslips were coated with 0.1 mg mL-1 Poly-D lysine (Thermo Fisher Scientific, A3890401) at room temperature for 1 h in a 6-cm tissue culture plate.

Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor-orchestrated niche to shape early tumorigenesis
Article Snippet: .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. Next, we flowed imaging buffer into the sample and generated a low magnification map of the entire tissue using a 20X Plan APO objective (Nikon, MRD00205).

Inverted Microscopy:

Article Title: Epigenetic plasticity cooperates with cell-cell interactions to direct pancreatic tumorigenesis
Article Snippet: .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. Next, we flowed imaging buffer into the sample and generated a low magnification map of the entire tissue using a 20X Plan APO objective (Nikon, MRD00205).

Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor niche at the benign-to-malignant transition.
Article Snippet: Cleaned coverslips were coated with a silane layer to allow stabilization of a polyacrylamide gel during smFISH staining, following published protocols111: they were submerged in 0.1% (vol/vol) triethylamine (Millipore, TX1200) and 0.2% (vol/vol) allyltrichlorosilane (Sigma, 107778) in chloroform for 30 min at room temperature, washed once with chloroform, washed once with 100% ethanol and dried using nitrogen gas. .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. To prepare for staining individual samples, silanized coverslips were coated with 0.1 mg mL-1 Poly-D lysine (Thermo Fisher Scientific, A3890401) at room temperature for 1 h in a 6-cm tissue culture plate.

Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor-orchestrated niche to shape early tumorigenesis
Article Snippet: .. We placed the flow chamber for imaging on a Nikon Ti2 inverted microscope using the FCS2 stage adapter (Bioptechs, 060319-2-2611), and used our fluidics system to flow in 20X SSC into the sample in order to eliminate bubbles in the tubbing and chamber. .. Next, we flowed imaging buffer into the sample and generated a low magnification map of the entire tissue using a 20X Plan APO objective (Nikon, MRD00205).

Microscopy:

Article Title: Fast Relaxation Imaging in living cells.
Article Snippet: This protocol describes the technique of Fast Relaxation Imaging (FReI) as applied to protein folding inside living cells.. The required modifications of a fluorescence microscope by addition of a diode laser temperature jump source, a yellow/blue switchable lightemitting diode source, and a two-color CCD camera to collect movies of protein dynamics inside cells are discussed.. A description of how proteins are labeled for imaging, how cells are prepared for imaging, and how imaging of kinetics inside cells with millisecond time resolution is obtained, along with the complementary in vitro experiments, is also provided.



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